« Mike Goikhberg Bacterial Transformation Lab Report Introduction Bacterial transformation is the transforming of a bacterial cell using plasmids spliced with ...» Document abstract
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Language : english
medical studies
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23/10/2007
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SummaryBacterial transformation is the transforming of a bacterial cell using plasmids spliced with various types of DNA from other cells (Miyazaki, 201). Instead of a nucleus with chromosomes, bacterial cells have one large circular chromosome in their cell. They also have much smaller rings of DNA throughout their cytosol. These smaller DNA rings are the plasmids which one can manipulate and introduce into cells for transformation to occur. (Miyazaki, 203)
One example of such a manipulated plasmid is the pGLO plasmid. This is the plasmid that we used in this lab. We introduced this plasmid into E. Coli bacteria using the heat shock method. This method involves placing the transformation mixture with the bacteria and the plasmid into ice and then into a hot water bath several times. We also made use of a CaCl2 solution which made the cell walls of the cells even more permeable to the plasmids.
One example of such a manipulated plasmid is the pGLO plasmid. This is the plasmid that we used in this lab. We introduced this plasmid into E. Coli bacteria using the heat shock method. This method involves placing the transformation mixture with the bacteria and the plasmid into ice and then into a hot water bath several times. We also made use of a CaCl2 solution which made the cell walls of the cells even more permeable to the plasmids.
Table of Contents
- One example of such a manipulated plasmid is the pGLO plasmid. This is the plasmid that we used in this lab.
- The hypothesis was that bacteria on a plate with arabinose would fluoresce if they had been transformed, and that bacteria on a plate with ampicillin would still grow if they were transformed.
- First, we observed the plasmid mixture under a UV light, and noted that it did not fluoresce.
- The plate labeled DNA+/LB/amp/ara had many white colonies on it.
- The DNA-/LB/amp plate was the most puzzling one.
- In conclusion, our experiment mostly confirmed our hypotheses and predictions.
« settings. . Bacterial Transformation Introduction The process of genetic transformation is an important tool used in biotechnology. It ...» Document abstract
$1.95
2 word format
Language : english
medical studies
case study
date published
09/10/2007
review : not yet assessed
level : General public
requested 5 times
SummaryThe process of genetic transformation is an important tool used in biotechnology. It has been used in agriculture to create plants that are resistant to different kinds of conditions. In medicine it is being used to try and create bacteria that produce insulin which can be used to treat diabetes. Transformation can also e used to give bacteria the traits to digest oil, which then can be used to clean up oil spills. In nature transformation also accurse by itself, when suddenly bacteria become resistant to antibiotics, when they are introduced to it over and over. This happens because bacteria naturally share their DNA with each other which lets them adapt to the environment.
Table of Contents
- The process of genetic transformation is an important tool used in biotechnology.
- In our experiments we had several variables that were available because of the pGLo plasmid.
- Our group obtained two Falcon tubes which we labeled +DNA for one and the other –DNA.
- The –DNA agar plates contained bacteria that were not introduced to the pGLo plasmids.
- The plate which had the arabinase had 85% of the colonies glowing under a UV light.
- Based on what we have done so far other experiments might be interesting to conduct based on similar settings.
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