Bacterial Transformation Lab Report
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document in english
medical studies
case study
date published 23/10/2007
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level : General public
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SummaryBacterial transformation is the transforming of a bacterial cell using plasmids spliced with various types of DNA from other cells (Miyazaki, 201). Instead of a nucleus with chromosomes, bacterial cells have one large circular chromosome in their cell. They also have much smaller rings of DNA throughout their cytosol. These smaller DNA rings are the plasmids which one can manipulate and introduce into cells for transformation to occur. (Miyazaki, 203)
One example of such a manipulated plasmid is the pGLO plasmid. This is the plasmid that we used in this lab. We introduced this plasmid into E. Coli bacteria using the heat shock method. This method involves placing the transformation mixture with the bacteria and the plasmid into ice and then into a hot water bath several times. We also made use of a CaCl2 solution which made the cell walls of the cells even more permeable to the plasmids.
One example of such a manipulated plasmid is the pGLO plasmid. This is the plasmid that we used in this lab. We introduced this plasmid into E. Coli bacteria using the heat shock method. This method involves placing the transformation mixture with the bacteria and the plasmid into ice and then into a hot water bath several times. We also made use of a CaCl2 solution which made the cell walls of the cells even more permeable to the plasmids.
Table of Contents- One example of such a manipulated plasmid is the pGLO plasmid. This is the plasmid that we used in this lab.
- The hypothesis was that bacteria on a plate with arabinose would fluoresce if they had been transformed, and that bacteria on a plate with ampicillin would still grow if they were transformed.
- First, we observed the plasmid mixture under a UV light, and noted that it did not fluoresce.
- The plate labeled DNA+/LB/amp/ara had many white colonies on it.
- The DNA-/LB/amp plate was the most puzzling one.
- In conclusion, our experiment mostly confirmed our hypotheses and predictions.
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