«GENES AND CHROMOSOMES As a first approximation, genes can be defined as stretches of DNA that encode a single protein or a single functional RNA, such as an ...» Document abstract
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6 word format
Language : english
medical studies
research papers
date published
26/11/2007
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level : Advanced
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SummaryAs a first approximation, genes can be defined as stretches of DNA that encode a single protein or a single functional RNA, such as an rRNA or tRNA. There are exceptions to this rule because there are mechanisms, such as alternative splicing of the primary RNA transcript into different mRNAs, that may intervene between a given gene and a finished protein. As a result, in some cases a single gene may actually encode multiple proteins.
«Instead of a nucleus with chromosomes, bacterial cells have one large circular chromosome in The pGLO plasmid had two core genes on it that would allow us to ...» Document abstract
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2 word format
Language : english
medical studies
case study
date published
23/10/2007
review : not yet assessed
level : General public
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SummaryBacterial transformation is the transforming of a bacterial cell using plasmids spliced with various types of DNA from other cells (Miyazaki, 201). Instead of a nucleus with chromosomes, bacterial cells have one large circular chromosome in their cell. They also have much smaller rings of DNA throughout their cytosol. These smaller DNA rings are the plasmids which one can manipulate and introduce into cells for transformation to occur. (Miyazaki, 203)
One example of such a manipulated plasmid is the pGLO plasmid. This is the plasmid that we used in this lab. We introduced this plasmid into E. Coli bacteria using the heat shock method. This method involves placing the transformation mixture with the bacteria and the plasmid into ice and then into a hot water bath several times. We also made use of a CaCl2 solution which made the cell walls of the cells even more permeable to the plasmids.
One example of such a manipulated plasmid is the pGLO plasmid. This is the plasmid that we used in this lab. We introduced this plasmid into E. Coli bacteria using the heat shock method. This method involves placing the transformation mixture with the bacteria and the plasmid into ice and then into a hot water bath several times. We also made use of a CaCl2 solution which made the cell walls of the cells even more permeable to the plasmids.
« A plasmid contains genes normally not essential for cell growth or survival and We then separated the cell walls and chromosomes by spinning them in the ...» Document abstract
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2 word format
Language : english
biology
presentation
date published
02/10/2007
review : not yet assessed
level : General public
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SummaryDNA technology is vital to our technological progress as a society. Without such things as gene therapy which is part of DNA technology many medical feats would not have been accomplished. DNA manipulation is used in many aspects of life, ranging from farms to hospitals and the gene cloning we attempted in our lab was but a precursor to all these great advances.
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